2018-09-26 09:21:502025-05-25 02:12:47

locus

BSU06240

BSU_06240

geneLength

1038

1041

outlinks

bsu

BSU06240

BSU_06240

Gene

Coordinates

677,911 → 678,951

677,911 678,951

The protein

Catalyzed reaction/ biological activity

formation of butanediol from acetoin [Pubmed|18820069]

(R,R)-butane-2,3-diol + NAD⁺ --> (R)-acetoin + H⁺ + NADH (according to UniProt)

The protein

Structure

[PDB|4EJ6] (from ''Sinorhizobium meliloti'', 33% identity)

[PDB|6IE0]

Biological materials

Mutant

MGNA-C219 (ydjL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2217 NBRP B. subtilis, Japan]

BKE06240 (Δ[[gene|bdhA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGATTACCACTCCTATA, downstream forward: _UP4_TAATTTGAAACCAAAAAGAA

BKK06240 (Δ[[gene|bdhA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGATTACCACTCCTATA, downstream forward: _UP4_TAATTTGAAACCAAAAAGAA

MGNA-C219 (ydjL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2217 NBRP B. subtilis, Japan]

BKE06240 ([[gene|bdhA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGATTACCACTCCTATA, downstream forward: _UP4_TAATTTGAAACCAAAAAGAA

BKK06240 ([[gene|bdhA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGATTACCACTCCTATA, downstream forward: _UP4_TAATTTGAAACCAAAAAGAA

The protein

Protein family

[SW|zinc-containing alcohol dehydrogenase family] (according to UniProt)

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Cofactors]

NAD⁺ (according to UniProt)